Guoliang Yang

Department of Physics, Drexel, University, Philadelphia , PA  19014

Telephone: (215) 895-6669,  Fax: (215) 895-5934

Email: gyang@drexel.edu, Website: www.physics.drexel.edu/~gyang

  

 

Education

Southern Illinois University   Molecular Science/Physics                    Ph.D.               1992

Southern Illinois University   Physics                                                 M. S.               1987

Northeast Normal University            Physics                                     B. S.                1982

 

Professional Experience

Sept. 2005-Present   Associate Professor, Department of Physics, Drexel University.

Jan. 2000-May 2005 Assistant Professor, Department of Physics, Drexel University.

Jan. 2000-Present     Adjunct Associate Professor, School of Biomedical Engineering, Science and Health Systems, Drexel University.

June 2002-Present     Visiting Professor, Northeast Normal University , China .

June 2002-Present     Member, Nanotechnology Institute of Southeast of Pennsylvania .

March, 2000             Short-Term Visiting Professor, University of Bologna , Bologna , Italy .

1998-1999                Senior Research Associate, Department of Molecular and Cell Biology, University of California at Berkeley .

1996-1998                Senior Research Specialist, Howard Hughes Medical Institute, University of Oregon .

1992-1996                Postdoctoral Research Associate, Institute of Molecular Biology , University of Oregon .

1984-1992                Research/Teaching Assistant, Department of Physics, Southern Illinois University .

1982-1984                Physics Instructor, Department of Physics, Northeast Normal University , Changchun , China .

 

Honors, Memberships and Professional Activities

2001                         Antelo Devereus Award for Young Faculty, Drexel University .

2002, 2004               Drexel and MCP Hahnemann Universities Synergy Grant Awards.

1996 -                       Member, American Physical Society.

2000 -                       Member, American Biophysical Society.

2005                         NIH Special Emphasis Panel, (ZRG1 MOSS-E (02)).

2000-                        Grant reviewer, NIH, NSF and DOE.

 Publications

  1. "Quantitative measurement of DNA double strand breaks induced by hydroxyl free radicals and the effect of N-acetyl-L-cysteine by AFM",  Meihong Su, Yao Yang and Guoliang Yang, Submitted (2006). 
  2. "Patterning polyethylene oligomers on carbon nanotubes using physical vapor deposition" Lingyu Li, Yao Yang, Guoliang Yang, Xuming Chen, Benjamin S. Hsiao, Benjamin Chu, Jonathan E. Spanier and Christopher Y. Li, Nano Lett. Accepted for publication (2006).
  3. "A temperature control device for single molecule measurements using the AFM",  Yao Yang, Fan-Chi Lin and Guoliang Yang.  Rev. Sci. Instrum., In press (2006)
  4. "Depletion force from macromolecular crowding enhances mechanical stability of protein molecules" Guanghui Ping, Guoliang Yang, and Jian-Min Yuan, Polymer, 47, 2564-2570 (2006).
  5.  "Nanohole Fabrication using FIB, EB and AFM for Biomedical Applications", J. Zhou and G. Yang, International Journal of Precision Engineering and Manufacturing (In press, 2005).
  6. "Nanoelectrodes Fabricated from Electron Beam Deposited Carbon as Potential Electrochemical Neuronal Probes", Y. Wei, H. Yin, X. Cao, P. Ndungu, G. Yang, J.-C. Bradley, K. Pourrezaei, P. I. Lelkes and J. Z. Tsien, J. Biomed. Nanotechnol. 1, 336-340 (2005).
  7. "Graphite Nanoplatelet Reinforcement of Electrospun Polyacrylonitrile Nanofibers", J. Mack, L. Viculis, A. Ali, R. Luoh, G. Yang, H.T. Hahn, F. Ko and R. Kaner, Adv Mater. 17, 77-80 (2005).
  8.  "Atomic force microscopic and theoretical studies of poly-ubiquitin proteins",Y.L. Yeh, C.H. Chang, K.K. Liang , Y.J. Shiu, C. Su, M. Hayashi, C.-L. Chyan, G. Yang, Y. Moe, Y.J. Yan, S.H. Lin, Chem Phys Lett 399, 440-445 (2004).
  9.  "Reversible Mechanical Unfolding of Single Ubiquitin Molecules", C-L. Chyan, F-C. Lin, H. Peng, J-M. Yuan, C-H. Chang, S-H. Lin and G. Yang, Biophys. J. 87, 3995-4006, (2004).
  10. “Pathway shifts and thermal softening in temperaturecoupled forced unfolding of spectrin domains”, Richard Law, George Liao, Sandy Harper, Guoliang Yang, David W. Speicher, and Dennis E. Discher, Biophys J. 85, 3286-3293 (2003).
  11. “Electrospinning of Continuous Carbon Nanotube-Filled Nanofiber Yarns”, Frank K. Ko, Yury Gogotsi, Ashraf Ali, Nevin Naguib, Haihui Ye, Guoliang Yang, Christopher Li and Peter Willis,  Advance Materials, 15, 1161-1165 (2003).
  12. "Wrapping DNA double helix with plastics via self-assembly and photodecomposition of diazonium salt and diazoresin", Yen Wei, Jinyu Zheng, Hua Dong, Shan Cheng, Guoliang Yang, Kunyuan Qiu, Weixiao Cao, Zichen Li, , Polym. Prepr. (Am. Chem. Soc., Div. Polym. Chem.), Vol. 44(2), pp.182-183, 2003.
  13. “Structure And Properties Of Carbon Nanotube Reinforced Nanocomposites”, Frank K. Ko, Sakina Khan, Ashraf Ali, Yury Gogotsi, Nevin Naguib, Guoliang Yang, Christopher Li, Hideo Shimoda, Otto Zhou, Michael J. Bronikowski, Richard E. Smalley and Peter A. Willis, AIAA (American Institute of Aeronautics and Astronautics) 2002-1426.
  14. “The effect of poly(ADP-ribosyl)ation and Mg++ ions on chromatin structure revealed by scanning force microscopy”, M. d’Erme, G. Yang, E. Sheagly, F.o Palitti and C. Bustamante, Biochemistry, 40, 10947-10955(2001).
  15. “Production of WS2 Nanotubes by an Activation Method”, E. B. Mackie, D. H. Galvan, E. Adem, S. Talapatra, G. Yang, and A. D. Migone, Adv. Mater.,12, 495(2000).
  16. “Solid-State Synthesis and Mechanical Unfolding of Polymers of T4 Lysozyme”, G. Yang, C. Cecconi, W. Baase, I. Vetter, W. Breyer, J. Haack, B. Matthews, F. Dahlquist and C. Bustamante, Proc. Natl. Acad. Sci. U.S.A. 97, 139(2000).
  17. "Direct Observation of One-Dimensional Diffusion and Transcription by Escherichia Coli RNA Polymerase", M. Guthold, X. Zhu, C. Rivitti, G. Yang, N. Thomson, S. Kasas, B. Smith, P. Hansma and C. Bustamante, Biophys. J., 77, 2284(1999).
  18. “Facilitated Target Location on DNA by Individual E. coli RNA Polymerase Molecules Observed with the Scanning Force Microscope Operating in Liquid”, C. Bustamante, M. Guthold, X. Zhu and G. Yang, J. Bio. Chem., 274, 16665(1999).
  19. "Structural Studies of Chromatin with the Atomic Force Microscopy, C. Bustamante, G. Yang, S. Leuba, G. Zuccheri and B. Samori, Methods, 12, 73(1997).
  20. "Effects of Tip-Sample Forces and Humidity on the Imaging of DNA with a Scanning Force Microscope", G. Yang, J. Vesenka and C. Bustamante, Scanning, 18, 344(1996).
  21. "Scanning Force Microscopy of Chromatin Fibers: A New Approach to an Old  Problem", G. Yang, S. Leuba and C. Bustamante, J. Biomolec. Struct. Dynam , Biological Structure and Dynamics, Eds. R. H. Sarma and M. H. Sarma. Adenine Press, p155(1996).
  22. "Scanning Force Microscopy of Biological Macromolecules", C. Bustamante, D. Erie , and G. Yang, Nanofabrication and Biosystems: Integrating Materials Science, Engineering, and Biology, Eds. Hoch, Jelinski and Craghead, Cambridge University Press(1996).
  23. "Converting an Alcohol to an Amine in a Cationic Lipid Dramatically Alters the  Ultrastructure, Co-lipid Requirement, and Transfection Activity of DNA-Lipid  Complexes", C. Wheeler, L. Sukhu, G. Yang, Y. Tsai, C. Bustamante, P.Felgner, J. Norman and M. Manthorpe, BBA(Biochemica et Biophysica Acta), 1280, 1(1996).
  24. "Scanning Force Microscopy Study of Native and Linker Histone Depleted  Chromatin Fibers", G. Yang, S. H. Leuba, C. Bustamante, J. Zlatanova and K. van Holde, SPIE, 2384, 13(1995).
  25. "Role of Linker Histones in extended Chromatin Fiber Structure: Modeling and  Scanning Force Microscopy Studies Provide Insight into the Organization of  Chromatin Fibers", G. Yang, S. H. Leuba, C. Bustamante, J. Zlatanova and K. van Holde, Nature, Struct. Bio., 1, 761(1994).
  26. "DNA Bending by Cro Protein in Specific and Nonspecific Complexes:  Implications  for Protein Site Recognition and Specificity", D. Erie , G. Yang, H. Schultz, and C. Bustamante, Science, 266, 1562(1994).
  27. "Three-Dimensional Structure of Extended Chromatin Fibers as Revealed by  Tapping- Mode Scanning Force Microscopy", S. Leuba, G. Yang, C. Robert, B.  Samori, K. van Holde, J. Zlatanova, and C. Bustamante, Proc. Natl. Acad. Sci. U.S.A. 91, 11621(1994).
  28. "Adsorption Studies on Boron Nitride Substrates", P. Shrestha, M. Alkhafaji, M.  Lukowitz, G. Yang and A. Migone, Langmuir, 10, 3244(1994).
  29. "Linker DNA Accessibility in Chromatin Fibers of Different Conformations: A  Reevaluation", J. Zlatanova, S. H. Leuba, G. Yang, C. Bustamante and K. van Holde, Proc. Natl. Acad. Sci. U. S. A. 91, 5277(1994).
  30. "The Relationship between Thermal Diffusivity and Mean Free Path", G. Yang,  A. D. Migone and K. W. Johnson, Am. J. Phys., 62, 370(1994).
  31. "Humidity Effects on Atomic Force Microscopy of Gold-labeled DNA on Mica", J. Vesenka, S. Manne, G. Yang, C. Bustamante and E. Henderson, Scanning  Microscopy, 7, 781(1993).
  32. "Evidence of DNA Bending in transcription Complexes Imaged by Scanning Force  Microscopy", W. Rees, R. Keller, J. Vesenka, G. Yang and C. Bustamante, Science,  260, 1646(1993).
  33. "Scanning Force microscopy of Nucleic Acids and Nucleoprotein Assemblies", C. Bustamate, D. Keller and G. Yang, Curr. Opin. Struct. Bio., 3, 363(1993).
  34. "An Experimental Study of Some Thermophysical Properties of Solid Materials", G. Yang, Ph. D. dissertation, Southern Illinois University at Carbondale , 1992.
  35. "Heat Capacity and Thermal Conductivity of a Glass Sample", G. Yang, A. D. Migone and K. W. Johnson, Phys. Rev., B 45, 157(1992).
  36. "Automated Apparatus for the Determination of the Specific Heat and the  Thermal Diffusivity of Solids Using ac Techniques", G. Yang, A. D. Migone and  K. W. Johnson, Rev. Sci. Instrum., 62, 1836(1991).
  37. "Low Temperature Specific Heat Measurement of a Carbon-Carbon Composite", G. Yang and A. D. Migone, Cryogenics, 29, 1154(1989).
  38. "QNDE of a 3-D Carbon-Carbon Composite Using Photoacoustic Microscopy", T. Ahmed, K. W. Johnson, A. D. Migone, J. M. Monzyk, G. Yang, M. Zaman and J. Don, Rev. Prog. Quant. Nondestr. Eval., Vol. 7B(1988).
  39. "Characterization of Heterogeneous Materials Using Transmission Photoacoustic  Microscopy", T. Ahmed, J. W. Monzyk, K. W. Johnson, G. Yang and S. Goderya, Acoustic Imaging, Vol. 16, Ed. Lawrence K. Kessler, Plenun Press, New York and  London, p493(1988).
  40. "Photoacoustic Signal Enhancement", T. Ahmed, A. Biswas, J. M. Monzyk, G. Yang and K. W. Johnson, Appl. Phys. B43, 53(1987).

Invited Talks

  1. “The effects of macromolecular crowding on the mechanical stability of proteins”, Department of Physics, Wake Forest University, November 17, 2005.

  2. “Direct Measurement of the Energy Landscape Roughness of Protein Molecules”, Department of Physics, Fisk University, May 9, 2005.

  3. “Single Molecule Studies of Protein Folding”, Department of Physics, Northeast Normal University ( China ). July 12, 2004 .

  4.  “Single Molecule Studies of Protein Folding”, Department of Physics, Jilin University ( China ). July 9, 2004 .

  5. “Reversible Mechanical Unfolding of Single Ubiquitin Molecules”, Fourth Cross-Strait Workshop on Biology Inspired Theoretical Sciences, June 25-30, 2004 , Xiamen , China .

  6. "Protein folding and unfolding studied with single molecule AFM technique", Department of Physics, September 29, 2003 , Temple University , Philadelphia , PA.

  7. “Single Molecule Studies of Biological Macromolecules”, G. Yang, Department of Physics, Chung Yuan Christian University , Taiwan , July 15, 2002 .

  8. “Mechanical Unfolding of Single Protein Molecules”, G. Yang, Third Cross-Strait Workshop on Biology Inspired Theoretical Sciences, July 8-12, 2002, Hualien , Taiwan .

  9. “Protein Studies using the Atomic Force Microscopy”, Department of Physics, Northeast Normal University ( China ). June 27, 2002 .

  10. “Manipulating and Visualizing Biological Macromolecules with the Atomic Force Microscope”, Institute of Atomic and Molecular Sciences, Academia Sinica ( Taiwan ), December 19, 2001 .

  11. "Nanocomposite Thin Films Based on Arrays of Vinyl Monomer Modified Silica Nanoparticles", Invited Presentation with Graduate Travel Award ($500 to Ms. Shan Cheng), Summer Gordon Research Conference on Coatings and Films, July 15-20, 2001 at Colby-Sawyer College in New London , NH , (Yen Wei, Shan Cheng, Guoliang Yang, Kai Li, and Ray Yin).

  12. “Manipulating and Visualizing Biological Macromolecules with the Atomic Force Microscope”, Department of Chemistry, Drexel University , January 10, 2001 .

  13. “Manipulating and Visualizing Biological Macromolecules, One at a Time”.  Group on soft matter research, Department of Physics and Astronomy, University of Pennsylvania , November 30, 2000 .

  14. “Mechanical Unfolding of Proteins”, Department of Biochemistry, University of Bologna ( Italy ) March 22, 2000 .

 

Contributed Talks and Presentations

  1. “A temperature control device for single molecule measurements using the AFM”, (Poster), Y. Yang F-C. Lin and G. Yang, 50th Annual Meeting of the Biophysical Society, 02/18/2006-02/22/2006, Salt Lake City, UT.

  2. “Analysis of oxidative damage to plasmid DNA by atomic force microscopy”, (Poster), M. Su, Y. Yang and G. Yang, 50th Annual Meeting of the Biophysical Society, 02/18/2006-02/22/2006, Salt Lake City, UT.

  3. “Direct Measurement of Energy Landscape Roughness of Protein Molecules” (Poster), Y. Yang and G. Yang, 49th Annual Meeting of the Biophysical Society, 02/12/2005-02/16/2005, Long Beach, CA.

  4. “Direct Measurement of Energy Landscape Roughness of Protein Molecules” (Poster), G. Yang, Bio-Image Summer School, July11-22, 2005, Ecole Normale Supérieure, Paris.

  5. “Nanohole Fabrication Using FIB, EB and AFM for Biomedical Applications”, J. Zhou and G. Yang, The 2nd International Symposium on Nanomanufacturing, November 3-5, 2004, KAIST, Daejeon, Korea..

  6. “An Acoustic Tweezer For Manipulation Of Micro and Nanoparticles”. Ryszard Lec, Quliang Zang, Guoliang Yang, Johann deSA,  IEEE International Ultrasonics, Ferroelectrics, and Frequency Control 50th Anniversary Joint Conference , August 24-27 2004, Montréal, Canada.

  7. “Effects of Macromolecular Crowding on the Mechanical Unfolding of Ubiquitin”. H. Peng, F-C. Lin, J-M. Yuan and G. Yang,  March Meeting of the American Physics Society, March 22-26, 2004 , Montreal , Canada .

  8. “Unfolding of Individual Ubiquitin Molecules”. G. Yang, C-L. Chyan, H. Peng, F-C. Lin, J-M. Yuan, C-H. Chang and S-H. Lin  48th Biophysical Society Annual Meeting, February 14-18, 2004 , Baltimore , Maryland .

  9.  “Wrapping DNA double helix with plastics via self-assembly and photodecomposition of diazonium salt and diazoresin”. Wei, Yen; Zheng, Jinyu; Dong, Hua; Cheng, Shan; Yang, Guoliang; Qiu, Kunyuan; Cao, Weixiao; Li, Zi-Chen.  226th ACS National Meeting, September 7-11, 2003, New York , NY.

  10. “Mechanical unfolding of titin domains at low temperatures”, Ensheng Liu, Guoliang Yang,  Biophysical Society 47th Annual Meeting, March 1-4, 2003, San Antonio, TX.

  11. “Structure and Properties of Carbon Nanotube reinforced Nanocomposites” F. Ko, S. Khan, A. Ali, Y. Gogotsi, N. Naguib and G. Yang,  43rd AIAA/ASME/ASCE/AHS/ASC Structures, Structural Dynamics, and Materials Conference, April 22-25, 2002, Denver, Colorado.

  12. “Mechanical unfolding of titin domains at low temperatures”. E. Liu and G. Yang,  March Meeting of the American Physics Society, March 18 - 22, 2002, Indianapolis,  Indiana.

  13.  “Mechanical Unfolding Of Individual T4 Lysozyme Molecules”, G. Yang, C. Cecconi, W. A. Baase, B. W. Matthews, and C. Bustamante,  Poster Presentation at the 2001 Biophysical Society Meeting, February 17-21, 2001, Boston , MA.

  14.  "Nanocomposite Thin Films Based on 2-D Arrays of Vinyl Monomer Modified Silica Nanoparticles" Y. Wei, S. Cheng, G. Yang, K. Li and R. Yin. Summer Gordon Research Conference on Coatings and Films, July 15-20, 2001, New London, NH.

 

Research Grants

1. “Single molecule studies of protein unfolding/refolding”.

NIH (GM071793-01),                    08/01/04 – 07/31/09               Role: PI

 

2.  Supplement to “Single molecule studies of protein unfolding/refolding”

      NIH (GM071793-02S1)                09/01/05 – 05/31/06              Role: PI

 

3.  "NER: Acoustic Wave Nanoparticle Analyzer"

      NSF (ECS-0508475)                07/15/2005 – 06/30/2006           Role: Co-PI.

 

4. “AFM applications in the development of Novel Biosensors”

Nanotechnology Institute of Southeast PA,   08/01/02 – 12/31/2004  Role: PI.

 

5. “An Acoustic Tweezer for Nanoparticle Manipulation “

NSF (NER 0304639)                08/01/03 – 07/31/05     Role: Co-PI.  (PI: R. Lec). 

 

6. “Carbon Nanotube Reinforced Carbon Fiber.”

DARPA        08/01/03 – 7/31/05                  Role: Co-PI (PI: F. Ko).

 

7. “Characterization of PCADM-1/DNA Interaction for the Development of a ‘DNA-Protein’ Binding Assay for Prostate Cancer”

Drexel University (Synergy Grant)    07/01/02 – 06/30/03  Role: Co-PI (PI: M. Stearns)

 

8. “Visualization of the Active Species of Homologous Recombination Machinery using Atomic Force Microscopy”.

Drexel University (Synergy Grant)    07/01/04 – 06/30/05       Role: Co-PI (PI: A. Mazin).

 

9. “Single-Molecule Studies of the Protein Folding Using Bateriophage T4 Lysozyme”

Drexel University (Devereux Award)            01/01/02- 12/31/02      Role: PI.

 

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