The method to obtain the curvatures of the dendrites along their length involves first obtaining the digital pictures, and then to trace (with the computer mouse) the dendrites in the picture.
The program allows for the individual trace of dendrites as shown in the picture. As an illustration of the method, the picture shows a partial tracing of the dendrites that are to the left of the plaque.
A sample of the interactive output of the program (updated for every "click") is given in the data for the curvature. Quantities such as the current length traversed by that particular trace, the x-y locations of the mouse, the local curvature, and the radius of gyration are interactively updated as the trace is performed.
In the same fashion, the (dystrophic) dendrites containing the tau protein can be traced, as illustrated in a partial trace of the picture below.